Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human BDNF. Standards or samples are added to the well by micro-ELISA and combined with specific antibodies.

There are many companies that sell thousands of immunosorbent assay kits (ELISAs) to researchers. Species include humans, mice, rats, rabbits, monkeys, pigs, and many more. You can also check out the bdnf elisa kits from various online sources.

As a professional manufacturer and supplier of ELISA kits, these kits are strictly quality checked to ensure the accuracy of the results.

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Biotinylation detection antibodies specific to human BDNF and horseradish-radish peroxidase (HRP) conjugate were then added to each microplate alternately and incubated. The free components will be removed. The substrate solution is added to each hole.

Only wells containing human BDNF, biotinylation detection antibodies, and the avidin-HRP conjugate appear blue. The enzyme-substrate reaction is stopped by adding a stopping solution and the color turns yellow.

Optical density (OD) was measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the human BDNF concentration. You can calculate the human BDNF concentration in a sample by comparing the OD of the sample with a standard curve.

Kit components and storage

The unopened kit can be stored at 2-8 ° C for 1 month. If the kit will not be used within one month, store the components separately after receiving the kit according to the following conditions.